Index
Fast chiral screening identification protocol
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Case study 1
2-(2-Nitro-1-phenyl-ethyl)-malonic
acid diethyl ester
Case study 2
2-Acetylamino-4,4,4-trifluoro-butyr
ic acid
Case study 3
N-FMOC-2-Acetylamino-4,4,4-trifluoro-butyr
ic acid
Case study 4
1-(3,5-Dimethyl-pyrazol-1-yl)-propa
n-2-ol
Case study 5
cyclohexane-1,2-di amine derivative
Case study 6
N-Chroman-2-ylmethyl-benzamide
ChirBase Real Case Study
Practical example 2
Sub-structure search in Chirbase using following query structure built in ISIS/Draw:
ChirBase search results : 30 hits
- 14 hits on CHIRALPAK AD chiral selector - (1.11 < alpha < 3)
- 16 hits on CHIRALPAK AS chiral selector - (1.13 < alpha < 6.15)
The mobile phase used for these separations was always a mixture : alkane (80-96%)/ alcohol (20-4%)/ trifluoroacetic acid (0.1%)
.
Experimental results:
Chiral screening on these 2 columns allows separation on the following chiral selector:
- CHIRALPAK AD (alpha = 1.15)
mobile phase : hexane / alcohol / TFA (90/10/0.1)